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Old 05-10-2007, 10:38 PM   #1
ABBAS EL-TA'ALU
 
Default EFFECT OF MECHANICAL STRETCHING ON COLLAGEN & GAG SYNTHESES IN CONNECTIVE TISSUE

KOT, Y.G & EL-TA'ALU,
A. B

COMPARATIVE STUDY OF THE EFFECT OF MECHANICAL STRTCHING ON COLLAGEN
AND GLYCOZAMINOGLYCAN SYNTHESES IN THE CONNECTIVE TISSUE.

Department of
Biochemistry,
V. N. Karazin Kharkov National University, Svavody Square № 4, Kharkov
- 61077, Ukraine.
Paper presented in the III International Students' and Post -
graduates' Scientific Comference,
"The Young and Advancement", Biology. 23-27th April, 2007.
Ukraine: Ivan Frank L'viv
National University. ЗВЕ*НИК ТЕЗ, Pp. 68-69.

The study was in vitro carried out using the skin of 3-months old
male Wistar rats. Skin samples were incubated for six hours, at 37 oC
in Ringer-Kreb's solution, made up of 9.0752 gm of Tris HCl, 8.2998 gm
Nacl, 0.5206 gm Kcl, 2.2222 gm Mgcl2, 0.4333 gm NaHCO3; 5.4 gm
glucose. In this process, static mechanical stretching (load) of
(0-3.3) x 105 H/M2 was applied to the samples.
After incubation, samples were treated with liquid nitrogen, cut into
pieces and type I collagen in them was extracted using 1M solution of
Nacl. The obtained extract, after fitering, was passed through
Sephadex G-25.
Intensity of collagen synthesis in samples was determined by the
relative radioactivity of 3H- hydroxyproline, and its content by the
concentration of hydroxyproline.Tunicamicin was added to inhibit the
glycolysation of freshly synthesized collagen.
Glycozaminoglycans (GAGs) were isolated using ethanol, after
enzymatic treatment of samples with papain and collagenase I. Obtained
GAGs were afterwards fractionated by a modification of the method
Schiller et al (1961), by ion-exchange chromatography on Dowex 1 x 2 -
400, using 0.5 - 2M solutions of Nacl.
Concentration of GAGs was determined by way of the relative
concentrations of glucoronic and iduronic acids, while extent
isolation of thier separate fractions by using standard samples of
GAGs.
It was found out that, together with induction of collagen synthesis,
mechanical stretching leads to significant accumulation of hyaluronic
acid and chondroitin sulphate, that of chondroitin-6-sulphate, on the
other hand, was insignificant.
It was therefore, established that, the mode of accumulation of type
I collagen and isolated GAGs, in response to the same signal, is
different: maximum increases in concentration of GAGs was observed
with less amount of mechanical stretching, instead of maximum
intensity of collagen synthesis.

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Old 05-05-2016, 09:04 PM   #2
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